FastQCFastQC Report
Wed 25 May 2016
SRR1780775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486841
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384157.890666562594359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA503.4744935E-642.0039441
GACGCTT350.001255110139.9996515
CCCGGTC552.9928962E-431.8146328
GGTCCTA1450.031.3822571
ATACTCG450.004304066331.11403519
TATCGGA450.004308396531.10764126
CCCCGGT604.980378E-429.166417
GCCTTAC1004.412177E-728.002631
CGTGATA808.667169E-526.24707430
ACCTTTG700.001225601824.9997842
TTTAGCG700.00122633624.99721126
TTCGTGA1001.3978166E-524.49726728
GTTACGA750.001830519223.33552619
GATTGCA901.9281075E-423.3307330
CCGTGCG1102.9215082E-522.2702439
TTTCGTG1304.3406053E-621.53605827
CCGGTCG1003.9320978E-420.9976589
ACGACTT850.003787865620.58805322
CTGGATA1205.709407E-520.41648745
TACTCGG1257.808898E-519.60184120
Produced by FastQC (version 0.11.5)