FastQCFastQC Report
Wed 25 May 2016
SRR1780775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486841
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT210894.331804428961406No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG56021.1506836934440607No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC17740.36439001645301033No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC7700.15816252123383198No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT6690.13741652818887481RNA PCR Primer, Index 19 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT5030.10331915348132142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24500.060.14771370
CGTCTTA552.2846507E-950.9027448
CGTATGA2050.049.511141
TATGCCG40850.049.00245743
CTCGTAT42250.048.6261539
CGTATGC42200.048.60082641
CCGTCTT37550.048.5558647
GTATGCC41250.048.52728342
TCGTATG43400.048.46667540
CGTCTTC39050.048.39344848
CACGTAT800.048.1239439
GCCGTCT38600.048.05146
TCTCGTA42900.047.9709738
ATCTCGT42450.047.9023637
ACGTATG950.047.89368440
ATGCCGT41300.047.87538544
TGCCGTC40950.047.6863645
AATCTCG43750.047.59895336
CTTGAAA41650.047.38904657
TCTGCTT37750.047.28372253
Produced by FastQC (version 0.11.5)