FastQCFastQC Report
Wed 25 May 2016
SRR1780774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855308
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615357.194484326114102No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG58090.6791705444120715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT9900.051.61602854
TGGTCGC10200.050.78121649
TCGCCGT10100.050.2503452
GTCGCCG10600.048.8620951
CGGTGGT10650.048.63553246
GATCTCG10750.048.18874441
TCGGTGG10700.048.08117745
CGCCGTA10700.047.7596853
CTCGGTG10600.047.55261244
AGATCTC10900.047.20723740
GTAGATC11500.046.26616338
CGTATCA11000.046.13624656
ATCTCGG11100.046.03861242
TCTCGGT11100.045.72595643
TAGATCT11750.044.38805839
CCGTATC11500.044.1303255
GTATCAT11850.042.82689757
GGTGGTC11850.042.82439447
GTGGTCG12000.042.2890948
ATCATTA13000.039.0406559
Produced by FastQC (version 0.11.5)