FastQCFastQC Report
Wed 25 May 2016
SRR1780774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855308
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT342234.001248672992653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94891.1094249089216983No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG73500.8593395595504778No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT55580.6498243907457898No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC21410.25031918326497593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG52350.060.91793470
AACTCGT1050.056.66988437
CGAATGC1000.056.0031841
TATGCCG67800.052.7487743
CGTATGC69700.052.5783141
TCGTATG70850.052.51533540
GCTCGTT200.00694665752.5091264
CTGTCGT200.00695147852.49991222
CTCGTAT69900.052.37779239
GCCGTCT65750.052.37401646
CCGTCTT65150.052.37291347
CGTCTTC65800.051.97103548
ATGCCGT68900.051.9574244
TCTCGTA70650.051.87131538
GTATGCC69250.051.84641642
ATCTCGT70350.051.84374237
TGCCGTC68350.051.81233245
CTGCTTG65050.051.24003254
CTTGAAA69250.051.15690257
CATCTCG70950.050.91198336
Produced by FastQC (version 0.11.5)