FastQCFastQC Report
Wed 25 May 2016
SRR1780773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113212
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5701950.364802317775506No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG48204.257499205031269No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT6220.5494117231388899No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG2660.23495742500794967No Hit
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC2450.21640815461258525No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG1520.1342613857188284No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1400.123661802635763No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1210.10687912942090945No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1190.10511253224039854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA455.456968E-1270.010612
AAGTATT150.00221837770.010627
GGCCGTA150.00221837770.010653
AGTATTC150.00221837770.010628
ACGGCCG150.00221837770.010647
GGATCTC150.002222269869.9796840
TGTCGCG150.002222269869.9796841
GCGCGTC308.302293E-658.3679586
CGCGTCT308.302293E-658.3679587
GCGTCTG308.324094E-658.3421678
CGGCGAG308.345944E-658.31640222
ATCAATA308.345944E-658.31640259
TGCGCGT252.3748263E-456.033245
TCACACC252.3852277E-455.9837421
CCAGGGA200.006919663452.5311663
AGGGACA200.006919663452.5311665
CCTTGCA200.006919663452.5311663
TTCAGTG200.006931776652.50795432
ATGGCCC200.006931776652.50795434
TATTCAG200.006931776652.50795430
Produced by FastQC (version 0.11.5)