SRR1780773_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780773_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113212
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	30995	27.377839804967667	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8043	7.104370561424584	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	6362	5.619545631205172	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	5015	4.429742430131082	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	1713	1.5130904851075857	No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT	1049	0.926580221177967	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	699	0.6174257145885595	No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT	560	0.494647210543052	RNA PCR Primer, Index 34 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT	519	0.45843196834257854	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT	355	0.31357099954068474	No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	350	0.30915450658940746	No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	287	0.25350669540331416	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT	283	0.24997350104229232	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT	271	0.2393739179592269	No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCG	268	0.2367240221884606	No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	247	0.21817475179309614	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT	246	0.21729145320284068	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT	206	0.1819595095926227	No Hit
ATTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT	202	0.17842631523160088	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT	198	0.1748931208705791	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT	170	0.15016076034342649	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGA	162	0.1430943716213829	No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC	158	0.1395611772603611	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT	150	0.1324947885383175	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	134	0.1183620110942303	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	129	0.11394551814295303	No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTCATGGGATCTCGTATGCCGT	128	0.11306221955269759	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC	125	0.11041232378193125	RNA PCR Primer, Index 34 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	125	0.11041232378193125	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGAATGCCGT	120	0.105995830830654	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT	116	0.1024626364696322	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGGGATCTCGTATGCCGT	116	0.1024626364696322	No Hit
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	116	0.1024626364696322	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT	116	0.1024626364696322	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGC	15	0.0022153764	70.034485	41
ACTCCGA	40	1.3278623E-10	70.034485	11
TCGAAAG	15	0.0022153764	70.034485	40
GGTAGTC	15	0.0022153764	70.034485	41
ATGTGAT	15	0.00222316	69.97262	32
GTCTACA	15	0.00222316	69.97262	49
TGTGCTT	15	0.00222316	69.97262	53
TCTACAG	20	7.913148E-5	69.97262	50
AGTCTTA	20	7.913148E-5	69.97262	48
CTTCTTG	15	0.00222316	69.97262	54
CAGCTAG	30	1.018725E-7	69.97262	54
CTACAGC	20	7.913148E-5	69.97262	51
TAGGATC	15	0.00222316	69.97262	33
AAGGAGC	15	0.00222316	69.97262	6
AAATGTA	15	0.00222316	69.97262	49
AGCTAGA	35	3.6852725E-9	69.97261	55
ATTATAC	915	0.0	67.00249	1
AGGGGGG	4070	0.0	66.21956	70
CTAATGG	60	0.0	64.16991	29
GGATATA	55	0.0	63.667713	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content