FastQCFastQC Report
Wed 25 May 2016
SRR1780772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679917
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG481267.078216900003971No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG40190.5911015609258189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT6400.050.3057954
TCGCCGT6350.050.15078752
CTCGGTG6650.049.9896544
TCTCGGT6800.049.9161443
TGGTCGC6650.049.47435849
CGGTGGT6750.049.2599346
TCGGTGG6950.048.8495945
GATCTCG6750.048.2228841
ATCTCGG6850.047.50841542
GTCGCCG6600.047.19761351
CGCCGTA7050.045.66766453
GTAGATC7300.045.0856938
AGATCTC7400.043.98708740
CGTATCA7350.043.3275556
TAGATCT7750.042.4553239
GTGGTCG8200.041.82983848
CCGTATC7800.041.27654655
GGTGGTC8550.040.52686747
ACCCGAA350.00125214740.02115637
GTATCAT8200.038.8362857
Produced by FastQC (version 0.11.5)