FastQCFastQC Report
Wed 25 May 2016
SRR1780772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679917
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT254813.7476633177284877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66280.9748248683295166No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT52480.7718589180738238No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG52180.7674466148073956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC16300.23973514414259386No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT150.00222230670.017521
AGGGGGG41750.060.9523870
ATCGTAT2050.056.35555639
AATATCG2250.052.9021236
ATCGTAA200.006943949552.51313439
ATATCGA200.006943949552.51313437
CGTCTTA407.4706804E-752.50926648
TATGCCG53000.052.28803643
TCGTATG54650.052.06157740
CGTATGC53850.051.9251841
CCGTCTT50750.051.8674147
CTCGTAT52750.051.83286739
GCCGTCT51500.051.7917446
CGTCTTC51350.051.74233648
GTATGCC54050.051.4017442
AATCTCG52950.051.37262336
TCTCGTA53300.051.36369338
CTTGAAA53650.051.2707657
ATCTCGT52900.051.2226437
TGCCGAC1300.051.15911545
Produced by FastQC (version 0.11.5)