FastQCFastQC Report
Wed 25 May 2016
SRR1780771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96045
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5735159.712634702483214No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG46944.887292415013795No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT5550.5778541308761518No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG3020.31443594148576187No Hit
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC2110.2196886875943568No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG1510.15721797074288094No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1240.1291061481597168No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1070.1114061117184653No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAG150.00221841670.002081
AAGATAC150.00221841670.0020834
AAGTGTA150.00221841670.0020834
GGTGGTA150.00221841670.0020847
CCCTCAG150.00221841670.0020848
CCGGCGA150.00221841670.0020821
CAGTATC308.320478E-658.3350755
AAAAAGG3800.056.18588670
GCCGGCG509.658834E-1056.00166720
AAAGGGG252.379621E-456.00166769
TCTTTAT252.379621E-456.0016671
CTTTATA252.3857603E-455.97252
CGCTGGC200.006917630352.5289416
GAAAGGG200.006917630352.5289468
GTAGCCG200.006917630352.5289451
GTGTCAG200.00693191552.50156457
TCGGCGG200.00693191552.50156452
TAGCCGT200.00693191552.50156452
TGAAGTG200.00693191552.50156432
CGAATCA200.00693191552.50156456