FastQCFastQC Report
Wed 25 May 2016
SRR1780771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96045
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2975830.98339320110365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78198.140975584361497No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG61306.382424904992451No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT54425.666094018428861No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC18351.9105627570409702No Hit
CTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT8220.8558488208652194No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC7150.7444427091467541No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT4710.4903951272840856RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT3440.358165443281795No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT3060.31860065594252696No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2940.30610651257223176No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT2900.3019417981154667No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2730.2842417616742152No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC2650.2759123327606851No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2440.25404758186266857No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT2370.2467593315633297No Hit
TCTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCG2320.24155343849237335No Hit
ATTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1940.2019886511531053No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT1900.19782393669634024No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT1870.19470040085376647No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA1760.18324743609766256No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT1750.1822062574834713No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1660.1728356499557499No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGGTTCCCATCTCGTATGCCGT1640.17075329272736736No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT1510.15721797074288094No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1460.15201207767192462No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCACGTATGCCGT1360.14160029153001197No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC1350.1405591129158207No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1310.13639439845905565No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT1310.13639439845905565No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1280.13327086261648186No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1140.11869436201780414No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGAATGCCGT1130.1176531834036129No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1050.10932375449008278No Hit
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1040.1082825758758915No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT1030.10724139726170025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC500.070.0839841
TCTTTAT1250.070.0839841
TCTTAAT252.8011418E-670.0839841
AAACATC150.00221272170.047446
CATCTGA150.002217303370.0109451
TATAAAC150.002217303370.010943
GACATCT252.818566E-670.0109449
AAGATGT150.002217303370.010945
GAACACG150.002217303370.0109418
AATACAA301.0132135E-770.010943
ATCGTAA150.002217303370.0109439
ATATCGA150.002217303370.0109437
GCCGACA252.818566E-670.0109446
ATCGAAT150.002217303370.0109439
ATTCAGC207.885593E-570.0109451
ACCGGAA150.002221892669.9744827
CCGGAAC150.002221892669.9744828
CTTTAAA252.8273116E-669.9744857
CAGAGAC150.002221892669.9744814
AGAGTAC150.002221892669.9744825
Produced by FastQC (version 0.11.5)