FastQCFastQC Report
Wed 25 May 2016
SRR1780770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756795
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG542797.172219689612113No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG36780.4859968683725447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC200.006951409452.49945432
GCCGTAT7200.051.04113854
GTCGCCG7600.048.81850451
CGGTGGT7550.048.6749946
TCGCCGT7600.047.897452
TGGTCGC7800.047.56675349
TCGGTGG7750.046.9672645
TCTCGGT7750.046.51257743
CTCGGTG7700.045.90558244
CGTATCA8300.044.6983356
GATCTCG8200.044.39271541
CGCCGTA8300.043.85496553
ATCTCGG8300.043.8520742
AGATCTC8300.043.43615740
GTAGATC8550.042.5754738
GGTGGTC9150.041.69631247
CCGTATC9000.041.22179455
TAGATCT9200.039.5674239
GTGGTCG9750.039.4893848
GGTCGCC9500.039.0548150
Produced by FastQC (version 0.11.5)