FastQCFastQC Report
Wed 25 May 2016
SRR1780770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756795
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT77511.0241875276660126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63990.8455394129189543No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT21510.2842249222048243No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG12800.16913430981970018No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT12740.16834149274242036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA207.925645E-570.0021856
AGGGGGG25950.057.45843570
CGTTTGC800.056.87677441
GGTACGT252.3878047E-456.012851
TGCCGTA7200.054.44254345
GCCGTAA4550.053.84427646
TATGCCG20650.053.55745343
GCCGTAT3600.053.47035246
TCGTATG16450.053.40592240
TCTCGTA20400.052.50510438
CGCGTAA407.475901E-752.50510439
ATCTCGT21600.052.33959237
AAGCCGT2550.052.1584944
CGTATGC16650.052.13375541
CTCGTAT18200.052.12045339
ATGCCGT21600.051.3673444
GCCGTCA6950.051.36511246
CATCTCG25300.051.3257136
GTATGCC22900.050.89341742
CATCTAG11900.048.82504736