FastQCFastQC Report
Wed 25 May 2016
SRR1780769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545337
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501229.191013996849655No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG41320.7576966169542869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT200.006948071552.5038554
TGGTCGC6450.049.92156249
TCGCCGT6400.048.1285352
CTCGGTG6550.048.0951344
CGGTGGT6650.047.89385646
GCCGTAT6450.047.7554454
ATCTCGG6800.047.35641542
TCGGTGG6750.047.18432245
GTCGCCG6900.047.17304651
GTAGATC7000.047.01207438
GTTGCAT305.869234E-446.67865440
TCTCGGT6900.046.6700943
TGTTACG451.6833001E-646.6700953
AGATCTC7100.045.36375840
GATCTCG7100.045.36375841
CGTATCA6900.044.1336756
CGCCGTA7000.044.00322753
GGTGGTC7400.043.9856847
CGACTTG404.589626E-543.7532158
TAGATCT7550.043.5873539
Produced by FastQC (version 0.11.5)