FastQCFastQC Report
Wed 25 May 2016
SRR1780769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545337
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT221644.064275851445986No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT70941.3008469991949931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69661.2773752743716271No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG48550.8902751876362689No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC15130.2774431223261946No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT12830.23526736678420868No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5780.10598950740551255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT207.924833E-570.000140
AGGGGGG37450.061.22064670
CGTATGA2050.054.6342241
CGTCTTA1100.054.0909848
TCGTTGT653.6379788E-1253.8412934
TGACGTC2400.052.50007245
ACGTATG608.367351E-1152.50007240
GACGATC407.476974E-752.50007229
ATCGTAT1000.052.5000739
CCGTCTT49050.051.8043547
CGTCTTC49950.051.7117848
CATCTCG52550.051.55097236
GCCGTCT50150.051.5055546
TATGCCG51550.051.4646643
TCGTATG54150.051.44974540
ATCTCGT52400.051.43136237
CGTATGC52650.051.3865841
CATCGTT750.051.333432
CTCGTAT53550.051.30725539
TCTCGTA52600.051.2358138