FastQCFastQC Report
Wed 25 May 2016
SRR1780768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73874
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4356458.970679806156426No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG38655.231881311422152No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT5100.6903646749871403No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG2100.284267807347646No Hit
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC1710.23147521455451175No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG1490.20169477759428214No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1360.1840972466632374No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.17191434063405256No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1010.13671927877196308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGA207.87967E-570.049
TCGACGT207.87967E-570.052
GAAGTGT252.8147388E-670.033
ACGTATC207.87967E-570.055
GTCGACG207.87967E-570.051
GTCTGGC252.8147388E-670.010
GACGTAT207.87967E-570.054
GCGCTGG150.002216647470.015
CGAATCA207.87967E-570.056
TATCATA207.87967E-570.058
CGACGTA207.87967E-570.053
CCTTATA150.002216647470.01
ATCATAA301.0110671E-770.059
AAGTGTA301.0110671E-770.034
GCCGAAT150.002216647470.054
CCGAATC207.87967E-570.055
GGTCGAC207.87967E-570.050
CTGGCGG308.303028E-658.33333218
CGCTGCG200.00692643452.516
TGCCGGC200.00692643452.519
Produced by FastQC (version 0.11.5)