FastQCFastQC Report
Wed 25 May 2016
SRR1780768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73874
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2522934.151392912256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65218.82719224625714No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG48796.604488724043642No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT42065.69347808430571No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC15912.1536670547147847No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC5010.6781817689579555No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT4840.6551696131250508TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3560.4819016162655332No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3440.46565774155995343No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT3360.4548284917562336No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3120.42234074234507407No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT2260.30592630695508566No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT2240.30321899450415574No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2200.29780436960229584No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT1640.22199962097625686No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATATCGTATGCCGT1450.19628015269242222No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT1410.1908655277905623No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCG1400.18951187156509733No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1340.18138993421230742No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT1300.17597530931044753No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC1300.17597530931044753No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC1270.17191434063405256No Hit
TCATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1220.16514605950672767No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1190.16108509083033273No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGTAAGATCTCGTATGCCGT1060.14348755989928796No Hit
ATAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1050.142133903673823No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTAAGCCGT980.1326583100955681No Hit
CTTATACACATCTCCGAGCACACGAGACGGAGTAAGATCTCGTATGCCGT970.13130465387010315No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATATCGTATGCCGT960.1299509976446382No Hit
TCTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCG940.1272436851937082No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC880.11912174784091832No Hit
ATTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT870.11776809161545335No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGA870.11776809161545335No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT830.11235346671359342No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAG820.11099981048812843No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGAATGCCGT800.10829249803719847No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT800.10829249803719847No Hit
ATTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT790.10693884181173349No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT740.10017056068440859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA207.8671816E-570.0223611
TATTCTG401.3278623E-1070.0223650
CGAGCAA207.8671816E-570.0223615
AAAGATC800.070.0223633
TCTTAAT353.6397978E-970.022361
ACACGTA150.00221383670.02235438
CTTTACA301.0088297E-770.0223541
CATCTCG150.00221383670.02235436
TTCTGAA252.8093855E-670.02235452
TCAACGA150.00221383670.02235411
ACATCTA252.8093855E-670.0223548
CCGTATT150.00221383670.02235447
TCTGAAT252.8093855E-670.02235453
CTACGAG252.8093855E-670.02235412
ATTCTGC252.8093855E-670.02235451
ATTCTGA150.00221383670.02235451
ATCAACG150.00221383670.02235410
CAAACGA252.8093855E-670.02235419
ATCTGCA252.8093855E-670.02235452
AACACGT150.00221383670.02235437
Produced by FastQC (version 0.11.5)