FastQCFastQC Report
Wed 25 May 2016
SRR1780767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72908
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4698964.44971745213145No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG46526.380644099412959No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT5580.7653481099467823No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG2450.3360399407472431No Hit
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC1810.2482580786744939No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG1420.19476600647391232No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100.15087507543753773No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG960.13167279310912383No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG840.11521369397048335No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.10835573599604982No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.1056125528062764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150.002208173770.0666257
TACGCGT150.002214200670.018535
TCTTTAT207.868631E-570.018531
AGCAGTG207.89545E-569.9705132
TGGCGGC207.89545E-569.9705119
GAAAGCA207.89545E-569.9705129
AAGCAGT207.89545E-569.9705131
TTATTAC150.002220239769.97050535
GTCTGAC150.002220239769.97050510
CAGAGTA150.002220239769.97050534
AGAGTAG150.002220239769.97050535
CGACGAG150.002220239769.97050522
TGCGGGC150.002220239769.97050519
CGTCTGA150.002220239769.9705059
CATGTCC150.002220239769.9705059
GCTGGCG308.2888855E-658.34877417
GTGGCGC252.373045E-456.01482812
GGTCTCG252.3811139E-455.97640241
GTCTCGG252.3811139E-455.97640242
AAGAAAG252.3811139E-455.97640227
Produced by FastQC (version 0.11.5)