##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72908 Sequences flagged as poor quality 0 Sequence length 76 %GC 85 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.187153673122292 32.0 27.0 32.0 13.0 32.0 2 29.05131124156471 32.0 32.0 32.0 13.0 32.0 3 28.96115652603281 32.0 32.0 32.0 13.0 32.0 4 28.216766335655894 32.0 27.0 32.0 13.0 32.0 5 28.926235804026994 32.0 32.0 32.0 13.0 32.0 6 32.91114829648324 37.0 32.0 37.0 13.0 37.0 7 32.62990343995172 37.0 32.0 37.0 13.0 37.0 8 32.971992099632416 37.0 32.0 37.0 13.0 37.0 9 33.13584243155758 37.0 32.0 37.0 13.0 37.0 10-11 32.76795413397707 37.0 32.0 37.0 13.0 37.0 12-13 32.55894414879026 37.0 32.0 37.0 13.0 37.0 14-15 32.89820732978548 37.0 34.5 37.0 17.5 37.0 16-17 33.483705491852746 37.0 34.5 37.0 20.0 37.0 18-19 33.077776101388054 37.0 32.0 37.0 17.5 37.0 20-21 33.4803930981511 37.0 34.5 37.0 17.5 37.0 22-23 33.567928073736766 37.0 34.5 37.0 20.0 37.0 24-25 32.96034042903385 37.0 32.0 37.0 13.0 37.0 26-27 33.58085532451857 37.0 34.5 37.0 20.0 37.0 28-29 33.32545125363472 37.0 34.5 37.0 17.5 37.0 30-31 33.194039062928624 37.0 34.5 37.0 13.0 37.0 32-33 33.32018434191035 37.0 34.5 37.0 22.0 37.0 34-35 33.08637598068799 37.0 34.5 37.0 17.5 37.0 36-37 33.889360563998466 37.0 37.0 37.0 24.5 37.0 38-39 34.04328057277665 37.0 37.0 37.0 24.5 37.0 40-41 33.38282493004883 37.0 34.5 37.0 20.0 37.0 42-43 33.260190925550006 37.0 34.5 37.0 17.5 37.0 44-45 33.25279118889559 37.0 34.5 37.0 17.5 37.0 46-47 33.48250534920722 37.0 34.5 37.0 20.0 37.0 48-49 33.894545180227134 37.0 37.0 37.0 24.5 37.0 50-51 34.234734185548916 37.0 37.0 37.0 27.0 37.0 52-53 33.47411120864652 37.0 37.0 37.0 17.5 37.0 54-55 33.637550063093215 37.0 37.0 37.0 20.0 37.0 56-57 33.49317633181544 37.0 37.0 37.0 17.5 37.0 58-59 32.9422491358973 37.0 34.5 37.0 13.0 37.0 60-61 32.86592692159982 37.0 34.5 37.0 13.0 37.0 62-63 32.266946014154826 37.0 32.0 37.0 13.0 37.0 64-65 31.857539638997093 37.0 29.5 37.0 13.0 37.0 66-67 31.9122592856751 37.0 32.0 37.0 13.0 37.0 68-69 32.18747599714709 37.0 32.0 37.0 13.0 37.0 70-71 32.457604103802055 37.0 32.0 37.0 13.0 37.0 72-73 32.86607779667526 37.0 32.0 37.0 13.0 37.0 74-75 32.62786662643332 37.0 32.0 37.0 13.0 37.0 76 32.811461019366874 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.5411746790806262 11101 2 -0.12336421953629895 11101 3 -0.4498342475798722 11101 4 -0.5326549955839752 11101 5 -0.9991910847196728 11101 6 1.6502644244299987 11101 7 0.013846389020734762 11101 8 1.3608814584175448 11101 9 1.4527920937168517 11101 10-11 0.09264683373201166 11101 12-13 0.40586739552119155 11101 14-15 0.49150670097230886 11101 16-17 -0.12977254307235597 11101 18-19 0.43006586218204745 11101 20-21 -0.2293139202875878 11101 22-23 0.9581764911888797 11101 24-25 1.947056864180432 11101 26-27 1.243463086044862 11101 28-29 1.0235225463144886 11101 30-31 1.1533818872456578 11101 32-33 0.296220005611346 11101 34-35 1.7398398437650116 11101 36-37 1.2618204387115313 11101 38-39 0.8709871154918716 11101 40-41 1.2891771741982154 11101 42-43 0.8609451668043704 11101 44-45 1.0268053099473846 11101 46-47 0.2539348835270516 11101 48-49 0.5693289881533019 11101 50-51 0.666000605269879 11101 52-53 0.7353952103872672 11101 54-55 0.2897755030611293 11101 56-57 0.33335250554591767 11101 58-59 1.2005426190165096 11101 60-61 0.6508164027195349 11101 62-63 0.24666944996273088 11101 64-65 -0.5743303906110917 11101 66-67 0.5299786269852831 11101 68-69 0.2912006142518919 11101 70-71 0.3062595051454977 11101 72-73 -0.5505922763857498 11101 74-75 -0.09351284321662234 11101 76 -3.368990964213058 11102 1 0.6845709054957183 11102 2 0.5936169125391721 11102 3 -0.10190971927798387 11102 4 -0.5654851842632205 11102 5 0.47477117943126856 11102 6 0.12913234895830072 11102 7 0.6002614833603559 11102 8 -0.23006193780886974 11102 9 1.11354681069799 11102 10-11 -0.222447505890635 11102 12-13 0.6056787162759107 11102 14-15 -0.49604046883901276 11102 16-17 -0.4275083921289635 11102 18-19 0.9972356735028001 11102 20-21 -0.02591769387249343 11102 22-23 -0.22446501824508402 11102 24-25 -0.01558464525353287 11102 26-27 -0.23144257433249749 11102 28-29 0.2540885840503364 11102 30-31 0.11413660422678618 11102 32-33 0.1779181188188872 11102 34-35 -0.3126129864236731 11102 36-37 0.6878581745605814 11102 38-39 -0.6582581675270021 11102 40-41 0.5137054760850077 11102 42-43 0.3424546007666436 11102 44-45 -0.48527016175072646 11102 46-47 -0.6187066259069098 11102 48-49 -1.3884068609033022 11102 50-51 -0.47154656454144117 11102 52-53 -0.8742274311221649 11102 54-55 0.42298305023093263 11102 56-57 1.2792015621496944 11102 58-59 -0.407193230040086 11102 60-61 0.1530805536629316 11102 62-63 -0.9397456443768917 11102 64-65 -0.7328209566488297 11102 66-67 -1.7756817503732165 11102 68-69 -0.46898806499338974 11102 70-71 -1.0286461552318613 11102 72-73 0.15280395002933744 11102 74-75 -0.4348335979336042 11102 76 -1.1452173793073968 11103 1 0.10655929446524226 11103 2 -0.7387488349209157 11103 3 0.14555036780474495 11103 4 -1.6126549955839735 11103 5 -0.20919108471967363 11103 6 -1.9666586524930771 11103 7 -0.241538226363879 11103 8 0.9208814584175471 11103 9 1.3504844014091617 11103 10-11 -0.028507012421840727 11103 12-13 -0.12182491217111391 11103 14-15 -0.15387791441230547 11103 16-17 -0.2743879276877479 11103 18-19 -0.5787802916641098 11103 20-21 -0.2770062279798893 11103 22-23 1.417407260419644 11103 24-25 0.1839799411035088 11103 26-27 -0.44461383703205826 11103 28-29 0.08083023862218397 11103 30-31 0.08184342570719849 11103 32-33 0.561989236380569 11103 34-35 0.19137830530347344 11103 36-37 0.4872050540961439 11103 38-39 0.43444865395340315 11103 40-41 -0.18966897964794072 11103 42-43 0.3451759360351474 11103 44-45 0.08911300225508256 11103 46-47 -0.04414203954986817 11103 48-49 0.1108674496917672 11103 50-51 1.7840775283467991 11103 52-53 0.46539521038727116 11103 54-55 -1.1036860354004148 11103 56-57 0.23566019785361192 11103 58-59 0.563234926708823 11103 60-61 0.29658563348876754 11103 62-63 -0.4440997808065035 11103 64-65 0.817977301696601 11103 66-67 -1.4661752191685693 11103 68-69 -1.3695686165173377 11103 70-71 -0.1937404948545023 11103 72-73 -0.011746122539598502 11103 74-75 -0.3254359201397037 11103 76 -1.710529425751517 11104 1 -0.4406435027375579 11104 2 0.6039085077364277 11104 3 0.9938021160564929 11104 4 0.30552682259784447 11104 5 0.7498998243712371 11104 6 -0.8533719392063617 11104 7 0.8283918435661874 11104 8 0.0481541856902723 11104 9 1.4109739118986724 11104 10-11 -0.4891713480861739 11104 12-13 0.2540492137030057 11104 14-15 0.09332488279049045 11104 16-17 0.4556820023821828 11104 18-19 0.3409749530911341 11104 20-21 0.0034133524396864345 11104 22-23 -0.4200053269929356 11104 24-25 0.3343295914531552 11104 26-27 -0.901082368500596 11104 28-29 -1.1381732187487614E-4 11104 30-31 0.3024727963365663 11104 32-33 0.10622000561134826 11104 34-35 0.9343852983104597 11104 36-37 0.73091134780244 11104 38-39 0.21189620640095796 11104 40-41 0.6255408105618443 11104 42-43 -0.1081457422865384 11104 44-45 -0.6350128718707921 11104 46-47 0.1857530653452315 11104 48-49 -0.6143073754830652 11104 50-51 0.22690969617896428 11104 52-53 0.9744861194781862 11104 54-55 -0.299315406029784 11104 56-57 -0.47482931263589734 11104 58-59 -0.11491192643803316 11104 60-61 -0.2910017790986501 11104 62-63 -0.3224214591281793 11104 64-65 0.14385142757072344 11104 66-67 1.1208877178943695 11104 68-69 -0.2569812039299251 11104 70-71 0.2653504142364085 11104 72-73 0.745771359977887 11104 74-75 -0.6507855704893508 11104 76 -1.985354600576695 11105 1 -0.2588253209193745 11105 2 -0.2606191214970828 11105 3 -0.7541479730700686 11105 4 1.7500901024552427 11105 5 0.7662991113587587 11105 6 0.07104873815548984 11105 7 -1.7438006698027948 11105 8 1.4306853799861727 11105 9 0.1457332701874421 11105 10-11 -0.425000225091523 11105 12-13 -0.7406031927141044 11105 14-15 0.6820949362664237 11105 16-17 -0.17408626856255438 11105 18-19 0.40104625433890817 11105 20-21 0.011078236575166045 11105 22-23 -1.2292744892032772 11105 24-25 -0.37333529268232013 11105 26-27 0.9515023017311321 11105 28-29 -1.010987257607077 11105 30-31 0.38161718136331046 11105 32-33 -0.10750548458473475 11105 34-35 -1.0201601562349936 11105 36-37 0.582604752437021 11105 38-39 -1.1358756296061685 11105 40-41 -0.32258753168413534 11105 42-43 0.16172948052986413 11105 44-45 -0.4218221410330045 11105 46-47 0.26864076587999364 11105 48-49 0.6591329097219258 11105 50-51 1.15756923272086 11105 52-53 0.18108148489707787 11105 54-55 0.38820687561014466 11105 56-57 0.2982544663302349 11105 58-59 1.1011308543106324 11105 60-61 -0.8680071266922305 11105 62-63 1.0415714107470393 11105 64-65 1.7237088250751782 11105 66-67 1.257429607377432 11105 68-69 1.1357104181734599 11105 70-71 0.15429872083176832 11105 72-73 1.5231332138103255 11105 74-75 0.33727147050886686 11105 76 -0.06271645440913431 11106 1 0.5139019518078989 11106 2 -0.4188187649908457 11106 3 -0.7880160657616884 11106 4 -1.885382268311247 11106 5 1.0408089152803264 11106 6 -0.048826484660907 11106 7 1.3738463890207342 11106 8 -0.30639126885517953 11106 9 0.7927920937168551 11106 10-11 0.5335559246410995 11106 12-13 0.8108673955211927 11106 14-15 -1.8453114808458722 11106 16-17 1.3375001842003655 11106 18-19 0.16597495309113697 11106 20-21 0.15568607971241732 11106 22-23 0.5004492184616041 11106 24-25 -0.5406704085468448 11106 26-27 -0.3510823685005917 11106 28-29 -0.3501138173218692 11106 30-31 -0.37707265820888836 11106 32-33 -0.45287090347956394 11106 34-35 -1.042887428962267 11106 36-37 1.0990931659842573 11106 38-39 0.6914416609464169 11106 40-41 -1.2290046439836075 11106 42-43 -0.11723665137743922 11106 44-45 1.3058962190382957 11106 46-47 0.5152985198906919 11106 48-49 0.4334198972442067 11106 50-51 -0.18445394018466743 11106 52-53 1.4653952103872712 11106 54-55 1.9688664121520318 11106 56-57 0.6456252328186451 11106 58-59 1.4032698917437898 11106 60-61 2.054452766355894 11106 62-63 -0.3156032773099966 11106 64-65 -1.3288758451565457 11106 66-67 -0.3700213730147226 11106 68-69 0.2930187960700721 11106 70-71 1.260804959690951 11106 72-73 -0.27922864002211867 11106 74-75 -1.7485128432166235 11106 76 -0.8171727823948736 11107 1 0.38403182193776786 11107 2 0.2337786376065587 11107 3 -3.1365009142465397 11107 4 -1.0412264241554006 11107 5 -1.5544291799577685 11107 6 -1.913545099379526 11107 7 0.2071797223540699 11107 8 1.5875481250842114 11107 9 -0.016731715806955094 11107 10-11 -0.3192579281727532 11107 12-13 0.3406293002830978 11107 14-15 -1.0027790133134076 11107 16-17 0.7768941235943103 11107 18-19 -0.049934137817956525 11107 20-21 -0.7074091583828235 11107 22-23 0.43389077690316213 11107 24-25 -0.5834193262957612 11107 26-27 0.10346308604486154 11107 28-29 0.40637968917162937 11107 30-31 -1.599475255611484 11107 32-33 -1.0437799943886574 11107 34-35 0.27745889138405744 11107 36-37 0.5146775815686695 11107 38-39 -0.5742509797462247 11107 40-41 -0.291775206754167 11107 42-43 -0.8585786427194364 11107 44-45 -0.8770042138621363 11107 46-47 -0.22225559266342287 11107 48-49 -0.91019482137051 11107 50-51 0.3550482243174997 11107 52-53 -0.03460478961272884 11107 54-55 -1.0354625921769696 11107 56-57 -1.03998082778741 11107 58-59 -0.690885952412053 11107 60-61 -0.5234693115661813 11107 62-63 -0.71333055003727 11107 64-65 -0.6124256287063297 11107 66-67 0.0704548174614672 11107 68-69 -0.3530851000338231 11107 70-71 -0.2175500186640278 11107 72-73 -1.0524970382905146 11107 74-75 0.3764871567833765 11107 76 -0.4870862023082978 11108 1 -0.28056445135415586 11108 2 0.876635780463701 11108 3 0.6171222741592572 11108 4 0.40908413485080786 11108 5 0.8451567413672834 11108 6 1.2406992070386949 11108 7 0.9825420411946482 11108 8 0.4860988497218983 11108 9 1.6623573111081598 11108 10-11 0.1709077032972246 11108 12-13 0.07717174334727872 11108 14-15 0.12455017923317513 11108 16-17 1.284140500405904 11108 18-19 0.5261528187037783 11108 20-21 0.20459912319068252 11108 22-23 0.8981764911888774 11108 24-25 0.19401338591955408 11108 26-27 0.8534630860448615 11108 28-29 1.5243921115318813 11108 30-31 0.4455558002891351 11108 32-33 0.7496982664809053 11108 34-35 -0.43320363449586097 11108 36-37 -0.43252738737542273 11108 38-39 -0.794230275812474 11108 40-41 1.676133695937338 11108 42-43 1.4509451668043738 11108 44-45 -0.44015121179174344 11108 46-47 1.0691522748313957 11108 48-49 0.7797637707619955 11108 50-51 1.1925223444003166 11108 52-53 0.29148216690900597 11108 54-55 0.08151463349590671 11108 56-57 1.2172655490241837 11108 58-59 1.217499140755649 11108 60-61 1.973425098371706 11108 62-63 1.0257998847453393 11108 64-65 0.6908870006932517 11108 66-67 0.7495438443765821 11108 68-69 -0.0518428640089752 11108 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21612 44-45 0.8859357447299985 21612 46-47 1.058282709614005 21612 48-49 1.2471550751098235 21612 50-51 1.464261474835098 21612 52-53 -0.02373522439533815 21612 54-55 0.5597755030611253 21612 56-57 -0.39143010314973736 21612 58-59 -0.07597912011392083 21612 60-61 -0.16787924945437993 21612 62-63 0.18884336300620674 21612 64-65 -0.4286782166980494 21612 66-67 -0.022195286058199315 21612 68-69 -0.4866254727046311 21612 70-71 0.8714768964498418 21612 72-73 -0.11667923290748661 21612 74-75 2.996052374174681 21612 76 3.077095992308685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 8.0 12 28.0 13 60.0 14 126.0 15 235.0 16 380.0 17 530.0 18 653.0 19 735.0 20 737.0 21 767.0 22 783.0 23 961.0 24 1033.0 25 1119.0 26 1272.0 27 1546.0 28 1710.0 29 2111.0 30 2626.0 31 3134.0 32 4043.0 33 5265.0 34 7277.0 35 11092.0 36 24676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 79.31994623213453 3.7061421556524836 3.285051984747483 13.688859627465503 2 76.09217474795967 3.7185378231945685 15.163569028187368 5.025718400658391 3 75.58058188502216 4.12614367429802 15.725435865077298 4.567838575602529 4 76.0821857683656 11.507653481099469 7.359960498162067 5.0502002523728535 5 75.30622042383925 6.826692270763322 15.3213085522255 2.5457787531719362 6 78.63824650920364 14.47343154198557 4.13409047266343 2.7542314761473676 7 77.46975391621629 6.304353790019477 3.9230748127623385 12.302817481001894 8 78.07755085245587 14.325099098851963 3.209568354204672 4.387781694487498 9 76.22829396757469 4.284969686994212 3.158862096398102 16.327874249033 10-11 77.64366200080927 9.200951917207892 8.481527456776238 4.673858625206603 12-13 76.86304094369385 4.758932857828682 9.356697071531444 9.021329126946027 14-15 82.69940333310473 7.965160139908099 4.413277552979905 4.92215897400727 16-17 81.90141762741158 5.2411749778817205 3.759764620354304 9.097642774352398 18-19 77.48074588685509 4.265737622845699 8.453292916269469 9.80022357402975 20-21 82.33216517045805 4.354892910782989 4.6772235671716516 8.635718351587307 22-23 82.12105833459064 4.185469159339981 3.588818631955779 10.104653874113597 24-25 78.42794340541394 9.134552736799511 3.610202388055607 8.82730146973095 26-27 82.53128964784145 8.798134622638274 3.9419812776463328 4.72859445187395 28-29 82.1549075547265 10.029077741811598 4.101058868711252 3.714955834750645 30-31 78.81919679596203 8.9249465079278 8.486037197564054 3.769819498546113 32-33 81.7027171602178 5.4048308118562005 8.945643079539687 3.9468089483863005 34-35 78.67870957122871 8.316187968068471 4.6580528351576 8.347049625545221 36-37 82.42701067835318 5.044270243949276 8.572859014189795 3.9558600635077394 38-39 82.98162715258584 4.330889564030642 9.434001083579654 3.253482199803858 40-41 82.15554488718195 8.910911460119333 5.304848775804129 3.6286948768945884 42-43 78.6089031842154 8.902498405491965 8.60965757511333 3.878940835179305 44-45 77.5734840275968 4.512598241595457 9.53543555488499 8.37848217592275 46-47 81.71798916398052 3.94005898086551 5.3171936081201565 9.02475824703381 48-49 83.37082504629312 3.8385570262670603 8.764145120362116 4.026472807077703 50-51 87.1003360537686 3.82895549002126 5.3274809683835125 3.743227487826624 52-53 78.61997544733178 4.206187546722082 8.985727904313178 8.188109101632959 54-55 82.15385459458348 4.162180326857002 4.583267609883893 9.100697468675632 56-57 81.95584634693331 4.243222297663381 4.292601965585114 9.508329389818186 58-59 78.3142776798513 8.488967839726751 8.910089779905487 4.286664700516457 60-61 77.12169529883757 5.316325480917601 9.455131502245996 8.106847717998834 62-63 77.18400658391057 9.484946162814621 8.646183389342294 4.684863863932515 64-65 77.0990219881212 9.437883214682524 9.747198332030232 3.7158964651660424 66-67 77.12334028311204 14.831147810819708 4.30429057390541 3.7412213321628447 68-69 77.06430197788934 15.30601047924725 3.7863824650920366 3.8433050777713773 70-71 77.46274082151945 15.132747611503191 3.7434072001261978 3.6611043668511623 72-73 78.10643988752486 14.616967286194363 3.59303202798162 3.683560798299156 74-75 80.61503010684845 12.004334288888584 3.7966176087344152 3.5840179955285505 76 86.90130026883195 5.657815328907664 3.947440610083942 3.4934437921764414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 1.0 14 0.5 15 3.0 16 3.5 17 2.0 18 3.5 19 10.0 20 15.0 21 15.0 22 15.5 23 27.0 24 38.5 25 39.0 26 46.0 27 56.0 28 76.0 29 93.0 30 105.0 31 137.0 32 191.0 33 225.0 34 226.5 35 262.0 36 323.0 37 350.0 38 399.0 39 484.5 40 599.0 41 1084.5 42 1492.0 43 2689.5 44 3493.0 45 2000.5 46 902.0 47 820.5 48 684.5 49 574.0 50 518.0 51 483.0 52 430.0 53 400.0 54 388.0 55 370.0 56 319.5 57 267.0 58 247.0 59 226.0 60 194.0 61 175.5 62 168.0 63 156.5 64 137.5 65 134.5 66 121.5 67 104.0 68 108.5 69 100.0 70 85.5 71 84.0 72 81.5 73 75.0 74 80.0 75 89.0 76 87.0 77 76.0 78 84.0 79 101.0 80 96.0 81 95.5 82 102.5 83 105.0 84 111.0 85 127.5 86 149.0 87 160.0 88 173.5 89 200.5 90 240.0 91 284.0 92 302.0 93 337.5 94 424.0 95 598.5 96 722.0 97 909.0 98 1670.5 99 24974.0 100 47703.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002743183189773413 2 0.00411477478466012 3 0.009601141164206945 4 0.0 5 0.00411477478466012 6 0.002743183189773413 7 0.00822954956932024 8 0.0013715915948867064 9 0.002743183189773413 10-11 0.0034289789872167663 12-13 0.00411477478466012 14-15 0.00411477478466012 16-17 0.006172162176990179 18-19 0.00205738739233006 20-21 0.00205738739233006 22-23 0.0013715915948867064 24-25 0.004800570582103473 26-27 6.857957974433532E-4 28-29 0.0 30-31 0.0 32-33 0.0013715915948867064 34-35 0.002743183189773413 36-37 0.004800570582103473 38-39 0.00205738739233006 40-41 0.00411477478466012 42-43 0.00205738739233006 44-45 0.0013715915948867064 46-47 0.00411477478466012 48-49 0.00411477478466012 50-51 0.00411477478466012 52-53 0.004800570582103473 54-55 0.00205738739233006 56-57 0.004800570582103473 58-59 0.0102869369616503 60-61 6.857957974433532E-4 62-63 0.00411477478466012 64-65 0.006857957974433533 66-67 0.005486366379546826 68-69 0.002743183189773413 70-71 0.008915345366763592 72-73 0.00411477478466012 74-75 0.0013715915948867064 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 72908.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.383222691611348 #Duplication Level Percentage of deduplicated Percentage of total 1 89.08552587309065 18.158501124705108 2 6.264719736222327 2.5539035496790476 3 2.1532871273803917 1.3167279310912383 4 0.928605073682794 0.757118560377462 5 0.39028329183769594 0.3977615625171449 6 0.1480384900074019 0.18105009052504525 7 0.09420631182289213 0.13441597629889726 8 0.060561200457573516 0.09875459483184286 9 0.08074826727676468 0.1481318922477643 >10 0.6729022273063724 2.686947934383058 >50 0.07401924500370095 1.0410380205190104 >100 0.026916089092254895 0.9299391013331871 >500 0.006729022273063724 0.7653481099467823 >1k 0.006729022273063724 6.380644099412959 >5k 0.0 0.0 >10k+ 0.006729022273063724 64.44971745213145 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 46989 64.44971745213145 No Hit CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG 4652 6.380644099412959 No Hit TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT 558 0.7653481099467823 No Hit CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG 245 0.3360399407472431 No Hit ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC 181 0.2482580786744939 No Hit TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG 142 0.19476600647391232 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 110 0.15087507543753773 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 96 0.13167279310912383 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 84 0.11521369397048335 No Hit GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 79 0.10835573599604982 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 77 0.1056125528062764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002743183189773413 0.0 2 0.0 0.0 0.0 0.002743183189773413 0.0 3 0.0 0.0 0.0 0.002743183189773413 0.0 4 0.0 0.0 0.0 0.002743183189773413 0.0 5 0.0 0.0 0.0 0.017830690733527187 0.0 6 0.0 0.0 0.0 0.01920228232841389 0.0 7 0.0 0.0 0.0 0.01920228232841389 0.0 8 0.0 0.0 0.0 0.02331705711307401 0.0 9 0.0 0.0 0.0 0.02468864870796072 0.0 10 0.0 0.0 0.0 0.02468864870796072 0.0 11 0.0 0.0 0.0 0.02468864870796072 0.0 12 0.0 0.0 0.0 0.02468864870796072 0.0 13 0.0 0.0 0.0 0.026060240302847423 0.0 14 0.0 0.0 0.0 0.02743183189773413 0.0 15 0.0 0.0 0.0 0.02743183189773413 0.0 16 0.0 0.0 0.0 0.02743183189773413 0.0 17 0.0 0.0 0.0 0.02743183189773413 0.0 18 0.0 0.0 0.0 0.03154660668239425 0.0 19 0.0 0.0 0.0 0.043890931036374606 0.0 20 0.0 0.0 0.0 0.04937729741592144 0.0 21 0.0 0.0 0.0 0.050748889010808144 0.0 22 0.0 0.0 0.0 0.06172162176990179 0.0 23 0.0 0.0 0.0 0.06172162176990179 0.0 24 0.0 0.0 0.0 0.06720798814944862 0.0 25 0.0 0.0 0.0 0.07543753771876886 0.0 26 0.0 0.0 0.0 0.07680912931365556 0.0 27 0.0 0.0 0.0 0.08503867888297581 0.0 28 0.0 0.0 0.0 0.09052504526252263 0.0 29 0.0 0.0 0.0 0.10012618642672957 0.0 30 0.0 0.0 0.0 0.11384210237559664 0.0 31 0.0 0.0 0.0 0.13715915948867066 0.0 32 0.0 0.0 0.0 0.15910462500685796 0.0 33 0.0 0.0 0.0 0.17967849893015855 0.0 34 0.0 0.0 0.0 0.20711033082789268 0.0 35 0.0 0.0 0.0 0.2331705711307401 0.0 36 0.0 0.0 0.0 0.28254786854666153 0.0 37 0.0 0.0 0.0 0.34975585669611015 0.0 38 0.0 0.0 0.0 0.4334229439841993 0.0 39 0.0 0.0 0.0 0.5541230043342295 0.0 40 0.0 0.0 0.0 0.6679651067098261 0.0 41 0.0 0.0 0.0 0.8202117737422505 0.0 42 0.0 0.0 0.0 0.9806879903439951 0.0 43 0.0 0.0 0.0 1.1425357985406266 0.0 44 0.0 0.0 0.0 1.2920392823832776 0.0 45 0.0 0.0 0.0 1.4127393427333077 0.0 46 0.0 0.0 0.0 1.5224666703242442 0.0 47 0.0 0.0 0.0 1.6623690130026882 0.0 48 0.0 0.0 0.0 1.7638667910243047 0.0 49 0.0 0.0 0.0 1.8763373018050145 0.0 50 0.0 0.0 0.0 1.9792066714215175 0.0 51 0.0 0.0 0.0 2.0903055906073407 0.0 52 0.0 0.0 0.0 2.1986613266033905 0.0 53 0.0 0.0 0.0 2.289186371865913 0.0 54 0.0 0.0 0.0 2.3989136994568496 0.0 55 0.0 0.0 0.0 2.501783069073353 0.0 56 0.0 0.0 0.0 2.578592198387008 0.0 57 0.0 0.0 0.0 2.6403138201569103 0.0 58 0.0 0.0 0.0 2.728095682229659 0.0 59 0.0 0.0 0.0 2.829593460251276 0.0 60 0.0 0.0 0.0 2.931091238272892 0.0 61 0.0 0.0 0.0 3.0092719591814343 0.0 62 0.0 0.0 0.0 3.108026554013277 0.0 63 0.0 0.0 0.0 3.195808416086026 0.0 64 0.0 0.0 0.0 3.3082789268667363 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15 0.0022081737 70.06662 57 TACGCGT 15 0.0022142006 70.01853 5 TCTTTAT 20 7.868631E-5 70.01853 1 AGCAGTG 20 7.89545E-5 69.97051 32 TGGCGGC 20 7.89545E-5 69.97051 19 GAAAGCA 20 7.89545E-5 69.97051 29 AAGCAGT 20 7.89545E-5 69.97051 31 TTATTAC 15 0.0022202397 69.970505 35 GTCTGAC 15 0.0022202397 69.970505 10 CAGAGTA 15 0.0022202397 69.970505 34 AGAGTAG 15 0.0022202397 69.970505 35 CGACGAG 15 0.0022202397 69.970505 22 TGCGGGC 15 0.0022202397 69.970505 19 CGTCTGA 15 0.0022202397 69.970505 9 CATGTCC 15 0.0022202397 69.970505 9 GCTGGCG 30 8.2888855E-6 58.348774 17 GTGGCGC 25 2.373045E-4 56.014828 12 GGTCTCG 25 2.3811139E-4 55.976402 41 GTCTCGG 25 2.3811139E-4 55.976402 42 AAGAAAG 25 2.3811139E-4 55.976402 27 >>END_MODULE