FastQCFastQC Report
Wed 25 May 2016
SRR1780767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72908
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2312831.722170406539753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG729710.008503867888297No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG48256.617929445328359No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT33184.550940911834092No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC13771.888681626158995No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT11941.6376803642947277No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC5720.7845503922751962No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT5180.710484446151314No Hit
CTTATACACATCTCCGCGCCCACGAGACCATCCTGTATCTCGTATGCCGT4410.6048718933450375No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT4200.5760684698524168No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT3610.4951445657541011No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT3500.4800570582103473No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT2940.40324792889669175TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGA2060.28254786854666153No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2020.2770615021671147No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG1950.26746036100290776No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT1820.24962967026938057No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT1720.23591375432051354No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGAATCTCGTATGCCGT1620.22219783837164647No Hit
TCTTATACACATCTCCGCGCCCACGAGACCATCCTGTATCTCGTATGCCG1550.21259669720743954No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC1510.2071103308278927No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATTCCGT1380.1892796400943655No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGAATGCCGT1370.1879080484994788No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCAGT1260.17282054095572502No Hit
CTTATACACATCTCCGAGCCCACGAGCCCATCCTGTATCTCGTATGCCGT1250.17144894936083832No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT1170.16047621660174466No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG1110.15224666703242443No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG1080.1481318922477643No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC1030.14127393427333076No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC1000.13715915948867063No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGACGT950.13030120151423713No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACG930.1275580183244637No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCGCGTATGCCGT910.12481483513469029No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCCT890.12207165194491687No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCG860.11795687716025677No Hit
ATTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT840.11521369397048335No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC810.11109891918582324No Hit
ATTATACACATCTCCGCGCCCACGAGACCATCCTGTATCTCGTATGCCGT750.10286936961650299No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCAT730.10012618642672957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAT301.0034091E-770.074871
ATAATAC500.070.074871
GTATCAC353.6379788E-970.0267735
GTATCAA150.002213168270.0267735
TCACGTA301.0081931E-770.0267738
ATCACGT353.6379788E-970.0267737
CGAAAGA150.002213168270.0267741
ACGCGAC150.002213168270.0267722
CGACCAT150.002213168270.0267725
CTCGAAA207.86405E-570.02676439
AAGTATG207.86405E-570.02676440
CACGTAT252.8079703E-670.02676439
ACCCTCC401.3096724E-1070.02676427
TATCAAG207.86405E-570.02676436
GACCCTC401.3096724E-1070.02676426
ATCGTAA207.86405E-570.02676439
AGACCCT401.3096724E-1070.02676425
CAAGTAT207.86405E-570.02676439
CCGAAAT252.8194554E-669.97874547
GCCCAAG207.8908546E-569.9787418
Produced by FastQC (version 0.11.5)