FastQCFastQC Report
Wed 25 May 2016
SRR1780766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501143
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG461319.20515701107269No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG38760.7734319345975101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT200.006945254352.5086154
TTCGTGG200.00695348352.49289359
GTCGCCG6150.048.94134551
CGGTGGT6400.048.67014346
TCGGTGG6450.047.75023345
GCCGTAT5900.047.4654154
TCGCCGT6100.047.05689652
CTCGGTG6300.046.6743244
CGCGTCT451.6854465E-646.6603477
GTAGATC6700.046.49551838
TGGTCGC6400.046.4780849
GATCTCG6500.046.31528541
TCTCGGT6750.046.15571643
ATCTCGG6600.045.61353742
AGATCTC6600.045.60898640
TAGATCT6800.045.29702439
CGTATCA6650.043.1649756
CGCCGTA6650.042.6385753
GGTGGTC7200.042.2901647
GTATCAT7150.041.12562657
Produced by FastQC (version 0.11.5)