FastQCFastQC Report
Wed 25 May 2016
SRR1780766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501143
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT246844.925540215068354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63991.2768810499198833No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG51431.0262539833939615No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT50010.9979187577198524RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC20570.4104616845890295No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC150.002223578770.0046927
CGTCTTA700.065.0108448
AGGGGGG38750.060.62261670
CGCGTAA700.054.99819628
TGTCGAT200.00694791852.50351726
AATCGCG200.006953404352.49304210
CCGTCTT48650.050.8565747
GCCGTCT49000.050.85040346
TATGCCG50100.050.71183843
CGTCTTC49100.050.61944248
CGTATGC50550.050.4731241
CTTGAAA51550.050.4445157
TCGTATG51800.050.33618540
ATCTCGT50900.050.33233637
TGCCGTC50750.050.2002445
ATGCCGT51100.050.19882644
TCTCGTA51750.050.18192738
CTCGTAT51450.050.1344139
ACGTATG352.08618E-549.9983640
AATCTCG51050.049.97877536
Produced by FastQC (version 0.11.5)