FastQCFastQC Report
Wed 25 May 2016
SRR1780765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271259
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6377423.510372006090122No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG56292.075138520749542No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT7000.2580559539038336No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG3430.1264474174128785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT150.002222834670.0018443
TGGCGAC150.002224460769.9889419
CGCGTCT301.02245394E-769.988947
GTCGACG200.00694561652.5013848
GTCTGAC200.00694561652.5013810
GTGTCGG200.00694561652.5013842
GCCGTTG200.00694561652.5013828
GCGCGTC407.471117E-752.4917036
CACGAAG200.006950682552.49170323
TATGCGC200.006950682552.4917033
TGCGAAC200.006950682552.49170320
CACGTCT200.006950682552.4917037
TGGTCGC11100.050.13645649
TCGCCGT10500.050.00131652
CGACGAG352.085168E-549.992122
GCCGTAT10750.049.81526654
GTCGCCG10950.049.5446851
CGGTGGT11350.049.34050846
CGCCGTA10950.048.90539653
GATCTCG10750.048.83849341
Produced by FastQC (version 0.11.5)