FastQCFastQC Report
Wed 25 May 2016
SRR1780765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271259
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3456512.742434352408583No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91153.360257171190633No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG69212.551436081383475No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT62072.2882190084015646No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC19560.721082065479855No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC7640.2816496411178984No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT6950.25621269709023475No Hit
CTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCGT6780.24994562392399883No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT6580.24257259666960357No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4460.16441850777301398No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4140.1526216641659816No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC2890.10654024382601131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT353.6961865E-970.008346
CGTCTTA750.065.3290348
AGGGGGG51700.065.13344670
CATCTCG650.064.6230536
TGCCGTA401.0621079E-861.2572645
ATTATAC12700.061.188361
GTATTCT352.9687908E-759.9960549
AACGTAT308.357034E-658.3402539
GCCGACT1050.056.67338646
CATATGC252.386371E-456.0066451
AACTCGA252.386371E-456.0066437
TGACGAC452.690831E-854.45089745
ATCTCGT71150.054.36343437
CTTGAAA71450.054.03719357
TCTCGTA72300.054.03130338
CTCGTAT71800.054.01754439
AATCTCG71100.054.0078136
TCGTATG73650.053.99146340
CGTATGC72350.053.89719841
TATGCCG70650.053.85634643