FastQCFastQC Report
Wed 25 May 2016
SRR1780764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79056
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5393668.22505565674965No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG51656.53334345274236No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT6850.8664743978951629No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG2600.3288807933616677No Hit
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC2200.2782837482291034No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGG1650.20871281117182758No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1260.15938069216757741No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1060.1340821696012953No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950.12016798218984011No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940.11890305606152601No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG870.11004857316332727No Hit
CTTATACACATCTGCCGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG830.10498886865007084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAG150.002204362570.1026635
CAGAGTA150.00220991370.05826634
AGCAGTG150.00220991370.05826632
GTAAGCA150.00220991370.05826629
ATGCAGA150.00220991370.05826631
TGCAGAG150.00220991370.05826632
AAGCAGT150.00220991370.05826631
AGCCGAC207.875314E-570.0139219
GGTGTCG150.00221547470.0139241
CAGCCGA207.875314E-570.0139218
CTTTATA207.900061E-569.9696352
CTTTACA150.002221045369.9696351
ACGCAGC207.900061E-569.96963515
TTACACA150.002221045369.9696353
AGGTGGT150.002221045369.96963546
GACGCAG207.900061E-569.96963514
GCGCTGG207.900061E-569.96963515
CGTAACA150.002221045369.96963556
GCCGTAA150.002221045369.96963554
TCTTTAT207.900061E-569.9696351
Produced by FastQC (version 0.11.5)