FastQCFastQC Report
Wed 25 May 2016
SRR1780764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79056
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2866536.259107468123865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG839010.612730216555354No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG57827.313802873912163No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT46295.855343047965999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC16862.1326654523375836No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC6460.8171422788909128No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4910.6210787290022263No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4350.5502428658166363No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT3570.451578627808136No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT3530.44651892329487963TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT3410.4313398097551103No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT3190.40351143493219993No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT2500.3162315320785266No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2440.30864197530864196No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2300.2909330095122445No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC2140.27069419145921875No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA2010.2542501517911354No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT1950.24666059502125073No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1670.21124266342845577No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1650.20871281117182758No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1620.20491803278688525No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT1600.20238818053025703No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT1410.178354584092289No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1380.1745598057073467No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1350.17076502732240437No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT1280.16191054442420563No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGAATGCCGT1270.16064561829589152No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1260.15938069216757741No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG1170.14799635701275046No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1170.14799635701275046No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1160.14673143088443635No Hit
ATTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1100.1391418741145517No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1030.13028739121635297No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCGT990.12522768670309653No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCG960.12143290831815423No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT960.12143290831815423No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT960.12143290831815423No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG910.11510827767658369No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT890.11257842541995547No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACG880.11131349929164136No Hit
ATAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT830.10498886865007084No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT820.10372394252175672No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT820.10372394252175672No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT800.10119409026512852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGA150.002215155670.01646452
GAGAAAT150.002215155670.01646424
AATCTCG301.0103395E-770.01646436
TGCAAGA150.002215155670.01646455
ACGTAAG301.0103395E-770.01646440
CGGATGC150.002215155670.01646441
TATGAAT150.002215155670.01646453
AGAACTA150.002215155670.01646425
GACAACG150.002215155670.01646418
TAATCTC301.0103395E-770.01646435
GAACACG150.002215155670.01646418
AAGGAGG150.002215155670.01646470
GACCTTC150.002215155670.01646426
CGAGAAA150.002215155670.01646423
TTCAGAA150.002215155670.01646452
CGACCTA150.002215155670.01646425
AGACAAC150.002215155670.01646417
TGTCGTA301.0103395E-770.01646438
ACAACGA150.002215155670.01646419
GCAAGAA207.873901E-570.0164656
Produced by FastQC (version 0.11.5)