FastQCFastQC Report
Wed 25 May 2016
SRR1780763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622430
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6795510.917693555901868No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG59550.9567340905804668No Hit
TCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGT7080.1137477306685089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG207.9245016E-570.0022522
TCGCCGT10600.055.1432852
GCCGTAT10650.054.55574454
GTCGCCG11100.054.5425451
TGGTCGC11000.054.40646749
CGGTGGT11350.053.96649646
TCGGTGG11600.053.4068945
ATCTCGG11050.052.58087542
CGTATGT200.006947454552.5059135
CCGTAAG200.006951871352.497472
CTCGGTG11450.052.2766244
GTAGATC11450.051.665238
GATCTCG11350.051.50359341
CCGTATC11500.051.1320855
TCTCGGT11500.050.82772443
CGCCGTA11500.050.52336553
CAGTGTA12150.048.9727734
CGTATCA11750.048.85263456
GTGGTCG12550.047.96581648
TAGATCT12250.047.71965439
Produced by FastQC (version 0.11.5)