FastQCFastQC Report
Wed 25 May 2016
SRR1780763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622430
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT379246.092893980045949No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96731.554070337226676No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG77501.2451199331651752No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT66301.0651800202432402No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC23830.3828542968687242No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC8110.13029577623186545No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT8060.12949247304917821RNA PCR Primer, Index 19 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT7420.11921019231078195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG53750.061.41219370
TGACGAC352.9745206E-759.99772345
GCCGTAT553.6379788E-1157.27055746
TGCCGAC1450.055.51513745
ATATCGT1400.055.00675237
GCCGACT1450.053.10143346
ATCGTAT1400.052.50644739
CGAACTT200.006949378652.5022329
TATGCCG70600.051.9031343
CCGTCTT67750.051.7618847
CGTATGC71350.051.75412841
CGTCTTC68550.051.7194148
AATCTCG72650.051.699436
ATCTCGT72000.051.67995537
TCGTATG72800.051.6368140
GCCGTCT68750.051.56895446
CTCGTAT71950.051.32666439
GTATGCC71200.051.3182842
TCTCGTA72750.051.29152338
ATGCCGT71250.051.23314744