FastQCFastQC Report
Wed 25 May 2016
SRR1780762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59411.612903225806452No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG340.6647116324535679No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC270.5278592375366569No Hit
TCTTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGG130.2541544477028348No Hit
TCTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGT80.15640273704789834No Hit
GCGTCAGATGTGTATAAGAGACAGAGATTGCGCTCCGGATGGCGACGCCG60.11730205278592376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATG252.1717482E-455.99999627
CGGTGGT252.1717482E-455.99999643
GGTGGTC252.1717482E-455.99999644
CCAATGT200.006563731552.49999628
AATGTGT200.006563731552.49999630
TGTGTAG200.006563731552.49999632
CTCGGTG305.3432374E-446.66666441
ACCCAAT305.3432374E-446.66666426
GTGGTCG305.3432374E-446.66666445
GAGACCC305.3432374E-446.66666423
TGGTCGC350.001141900939.99999646
TCGGTGG350.001141900939.99999642
CATTAAA350.001141900939.99999658
TCGCCGT350.001141900939.99999649
TATCATT350.001141900939.99999658
CGACGAG350.001141900939.99999619
CGAGACC350.001141900939.99999622
GACCCAA350.001141900939.99999625
CGCCGTA350.001141900939.99999650
GTCGCCG400.00220129934.99999648