FastQCFastQC Report
Wed 25 May 2016
SRR1780762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1312.5610948191593352No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT1222.3851417399804498No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT190.37145650048875856Illumina PCR Primer Index 3 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG180.3519061583577713No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC160.3128054740957967No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.27370478983382207No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT110.21505376344086022No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT110.21505376344086022No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT90.17595307917888564No Hit
TCTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGA60.11730205278592376No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT60.11730205278592376No Hit
GGTACACAAGCACCCCACGGTGCCGTTCATACAGCGCCGCGCATGCCCAC60.11730205278592376No Hit
ATTATAAACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT60.11730205278592376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA406.111095E-752.49999656
TTGAAAA406.111095E-752.49999658
CTTGAAA406.111095E-752.49999657
TCTGCTT351.8072582E-549.99999653
TGCTTGA351.8072582E-549.99999655
CTGCTTG351.8072582E-549.99999654
TTCTGCT305.3432374E-446.66666452
CCGTCTT305.3432374E-446.66666447
GTCTTCT305.3432374E-446.66666449
GCCGTCT305.3432374E-446.66666446
CTTCTGC305.3432374E-446.66666451
ATGCCGT403.980808E-543.74999644
TGAAAAA502.8469658E-642.059
TATGCCG350.001141900939.99999643
AAGGGGG350.001141900939.99999670
AAAAGGG350.001141900939.99999668
TCTTCTG350.001141900939.99999650
CGTCTTC350.001141900939.99999648
TGCCGTC457.97786E-538.88888545
CGTATGC457.97786E-538.88888541