FastQCFastQC Report
Wed 25 May 2016
SRR1780761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT571.8440634098997088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG551.7793594306049825No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG110.3558718861209964No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT80.2588159171789065No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT60.19411193788417988No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT40.12940795858945325No Hit
CTTATACACATCTCCGAGCCCACGAGACCACATGGGATCTCGTATGCCGT40.12940795858945325No Hit
CAGCGTCAGATGTGTATAAGAGACAGGGGGCAGAGAGAGAGAAGTTGAAG40.12940795858945325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG252.2191743E-670.0000118
CGAGCCC252.2191743E-670.0000115
CCGAGCC252.2191743E-670.0000114
TCGTATG252.2191743E-670.0000140
GTATGCC206.730602E-570.042
ATCTCGT206.730602E-570.037
TGCCGTC206.730602E-570.045
TATGCCG206.730602E-570.043
TTCTGCT206.730602E-570.052
CCGTCTT206.730602E-570.047
AAGGGGG206.730602E-570.069
TCTGCTT206.730602E-570.053
TCTCGTA206.730602E-570.038
TGCTTGA206.730602E-570.055
ATGCCGT206.730602E-570.044
AAAGGGG206.730602E-570.068
GTCTTCT206.730602E-570.049
CGTATGC206.730602E-570.041
GCTTGAA206.730602E-570.056
CTGCTTG206.730602E-570.054
Produced by FastQC (version 0.11.5)