FastQCFastQC Report
Wed 25 May 2016
SRR1780760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633.2591826176927055No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT311.603724780134506No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG70.3621314019658562No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.20693222969477496No Hit
GCGTAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA30.1551991722710812No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA30.1551991722710812No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1551991722710812No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTATACACATCTCCGAGCCCAC30.1551991722710812No Hit
ATACTACAGCGATGGCTATTGAGGAGTATCCTGAGGCACTGTCTCTTATA30.1551991722710812No Hit
TTATTAGACATCCAAAAGTAGATGTCAAGTAAATAGTTGAATTTTTTTAT20.10346611484738748No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC20.10346611484738748No Hit
CGGAGATGTGTATAAGAGACAGGCTAAATAGGTTGTTGTTGATTTGGTTA20.10346611484738748No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG20.10346611484738748No Hit
GGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAG20.10346611484738748No Hit
CTTATAAACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT20.10346611484738748No Hit
CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT20.10346611484738748No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT20.10346611484738748No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT20.10346611484738748No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT20.10346611484738748TruSeq Adapter, Index 10 (95% over 21bp)
GAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTGT20.10346611484738748No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGATAATCTCGTATGCCGT20.10346611484738748No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA20.10346611484738748No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers