FastQCFastQC Report
Wed 25 May 2016
SRR1780759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6207
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2183.512163686160786No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC140.2255517963589496No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC80.1288867407765426No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG70.1127758981794748No Hit
CGCCAATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACATCGGGC70.1127758981794748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATTA200.00663197452.523
Produced by FastQC (version 0.11.5)