FastQCFastQC Report
Wed 25 May 2016
SRR1780758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6295.273748637545066No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG340.2850674939213549No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG220.18445543724322966No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC190.15930242307369832No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC200.00678500952.549
GTCGCCG200.00678500952.551
GGTGGTC200.00678500952.547
GTGGTCG200.00678500952.548
CTGCCGA900.004982054719.44444370
Produced by FastQC (version 0.11.5)