FastQCFastQC Report
Wed 25 May 2016
SRR1780758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1491.2492663704200553No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT200.16768676113020878No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.11738073279114615No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGC150.002171042470.08
AAGGGGG350.001206366340.070
GCGGCAT458.6647626E-538.88888532
CGGCATC458.6647626E-538.88888533
GAGACTT604.6975366E-429.16666624
CACGAGA755.1441868E-528.00000221
CCACGAG807.984735E-526.2520
CGAGACT700.001156671925.023
AAAAAAG750.001729102223.33333466
ACGAGAC800.002516664821.87522
CCCACGA900.004982054719.44444319
AAAAAAA4602.1684676E-48.36956664
Produced by FastQC (version 0.11.5)