FastQCFastQC Report
Wed 25 May 2016
SRR1780757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49632
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12442.506447453255964No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC150.002212340770.021
GTGCAAG150.002212340770.011
CTCCAGG200.0069130852.513
CACGAGA200.0069130852.57
TCCAGCG200.0069130852.519
CAAGATG200.0069130852.515
GCAATGC200.0069130852.530
GTCCCAA200.0069130852.537
TAGGCAC200.0069130852.548
GATCTGA200.0069130852.526
CTTATGA450.00426921331.1111134
ACGAGAC1159.896304E-418.26086870
CGAGACC1000.00956410717.569
GACGAGA1500.005830211614.069
CTGCCGA2407.930728E-411.66666670
GCTGCCG3104.5867622E-511.29032270
CGCTGCC3350.001179819.40298569
ACGCTGC3150.00758186738.88888868
GACGCTG3200.0086266358.7567
TGACGCT3250.0097938418.61538466
Produced by FastQC (version 0.11.5)