FastQCFastQC Report
Wed 25 May 2016
SRR1780757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49632
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2000.40296582849774343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1340.2699871050934881No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA150.002212340770.01
CATCTCG855.4569682E-1141.1764736
ATCTCGT953.6379788E-1240.52631837
GAAGCGA350.001244189740.021
CGCTCCA1150.039.56521631
ACGCTCC1150.039.56521630
TCTCGTA1007.2759576E-1238.538
CCATCTC1100.038.18181635
TATGCCG951.7826096E-1036.84210643
CGACGCT1300.035.00000428
GACGCTC1300.035.00000429
ATGCCGT904.327376E-935.00000444
TGCCGTC904.327376E-935.00000445
GTATGCC806.047776E-835.042
TCGTATG1102.1827873E-1135.040
GCTAGAA400.002397259235.056
GCCGTCT708.4789644E-735.046
GCTCCAT1251.8189894E-1233.632
CGTATGC957.3359843E-933.15789841
CTCGTAT1206.184564E-1132.08333239
Produced by FastQC (version 0.11.5)