FastQCFastQC Report
Wed 25 May 2016
SRR1780756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42169
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8952.122412198534468No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG520.12331333443999146No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG520.12331333443999146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCG150.00221002470.055
TAGATCT150.00221002470.039
GTATCAT252.3646172E-456.00000457
CTTCGAC200.00690589752.566
CGTATCA200.00690589752.556
CTCCACG200.00690589752.51
GTAGATC200.00690589752.538
CTCGGTG200.00690589752.544
TGACAGT200.00690589752.525
ATCCACC200.00690589752.511
GATTAAT200.00690589752.520
GCCGTAT200.00690589752.554
GCTCCAT200.00690589752.537
GAGGCAT200.00690589752.519
CAGAGAC305.814827E-446.66666447
TATCATT450.004261990531.11110958
AAATCGC500.00713306728.00000266
TACCATC500.00713306728.0000027
CTGCCGC657.8321656E-426.92307770
CGAGACC750.001804034823.33333468
Produced by FastQC (version 0.11.5)