FastQCFastQC Report
Wed 25 May 2016
SRR1780756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42169
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2080.4932533377599658No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT780.1849700016599872No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAA352.0531166E-550.03
AAGATCT604.7475623E-946.66666434
TCTTCTG602.521174E-740.83333250
GATCTCG602.521174E-740.83333236
CCGTCTT701.8537321E-840.047
AGATCTC556.5470467E-638.18181635
CGTCTTC654.7233152E-737.69230748
GTATGCC654.7233152E-737.69230742
GTCTTCT654.7233152E-737.69230749
TCTCGTA753.405694E-837.33333638
GCCGTCT753.405694E-837.33333646
TGCCGTC753.405694E-837.33333645
CTGCTTG601.1890306E-535.054
CGTATGC806.0108505E-835.041
CTCGTAT806.0108505E-835.039
TATGCCG708.4386556E-735.043
ATGCCGT806.0108505E-835.044
TCGTATG851.0242002E-732.94117740
ATCTCGT751.4471971E-632.66666837
CTTGAAA751.4471971E-632.66666857