FastQCFastQC Report
Wed 25 May 2016
SRR1780755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2483.6882807852468766No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC120.1784651992861392No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC70.10410469958358119No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA150.002141094369.9029852
TATAAGA150.002141094369.90298513
GATGTGT150.002141094369.9029857
CATCATC150.002141094369.90298521
ATGTGTA150.002141094369.9029858
GTATAAG150.002141094369.90298512
TCAGATG150.002141094369.9029854
AGATGTG150.002141094369.9029856
AGAGACA150.002141094369.90298517
GCGTCAG150.002141094369.9029851
GTCAGAT150.002141094369.9029853
TGTGTAT150.002141094369.9029859
GAGACAG150.002141094369.90298518
ATAAGAG200.00669229752.4272414
GTGTATA200.00669229752.4272410
CAGATGT200.00669229752.427245
TTTATGT200.00669229752.427244
TGTATAA200.00669229752.4272411
CTATTTA200.00669229752.427241
Produced by FastQC (version 0.11.5)