FastQCFastQC Report
Wed 25 May 2016
SRR1780754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3241.9826214661608128No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG210.1285032431770897No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG180.11014563700893402No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC170.10402643495288214No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAA150.002185656570.022
GAAATAT200.006830331452.50000435
CAGGCAT200.006830331452.5000044
TGCCGAC500.007007006627.99999870
TCTGACG900.005061278619.44444369
CTGACGC950.006917476718.42105370
Produced by FastQC (version 0.11.5)