FastQCFastQC Report
Wed 25 May 2016
SRR1780754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810.4956553665402032No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT400.24476808224207564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAG350.00121970940.062
GGGTGTT500.007007006627.9999987
Produced by FastQC (version 0.11.5)