FastQCFastQC Report
Wed 25 May 2016
SRR1780753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571.4285714285714286No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT180.4511278195488722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.17543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT70.17543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAATAAATCTCGTATGCCGT60.15037593984962408No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT40.10025062656641603No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT40.10025062656641603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG150.002063903570.0000168
GGGTGGT200.006452637752.5000041
Produced by FastQC (version 0.11.5)