FastQCFastQC Report
Wed 25 May 2016
SRR1780751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43782
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11382.599241697501256No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG510.11648622721666438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCAC150.002210588270.08
TCAGGGA150.002210588270.031
GTTCGAT150.002210588270.041
ACGGAGA150.002210588270.013
GAAGTCC150.002210588270.06
GGATTCT150.002210588270.025
GGTTCGA150.002210588270.040
CACGGAG252.3656164E-456.00000412
ATGGGTT200.006907645652.537
AGTCATA200.006907645652.510
GGCACGG200.006907645652.510
TTTCGTT200.006907645652.518
CACATGA200.006907645652.527
GCACGGA200.006907645652.511
CAATTTT200.006907645652.549
TCGATTC200.006907645652.543
GGCCCAA200.006907645652.51
ATTCATG200.006907645652.56
TTTGGAT200.006907645652.522
TGGATTC200.006907645652.524
Produced by FastQC (version 0.11.5)