FastQCFastQC Report
Wed 25 May 2016
SRR1780751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43782
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1800.4111278607646978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1310.2992097208898634No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA150.002210588270.047
AAAGAGC150.002210588270.054
ATCTATG200.006907645652.563
CATAGAC200.006907645652.52
CCCCCAG200.006907645652.564
AACTCGT200.006907645652.537
TAGTACT200.006907645652.54
ATCTCGT751.4551915E-1146.66666837
GAAAATC851.8189894E-1245.2941233
AATCTCG703.6743586E-1045.036
CGTATGC1000.042.00000441
TCTCGTA855.2750693E-1141.1764738
CTCGTAT1050.040.039
TATGCCG1050.040.043
GTGGAGT350.001242577440.010
GTATGCC1050.040.042
TCGTATG1050.040.040
TGCCGTC901.0004442E-1038.88888545
ATGCCGT1051.2732926E-1136.66666444
ACTTATG1151.8189894E-1236.5217427
Produced by FastQC (version 0.11.5)