FastQCFastQC Report
Wed 25 May 2016
SRR1780750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90301
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3306936.620856911883585No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG8940.9900222588897133No Hit
TCTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGT1150.1273518565685873No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATC1070.1184925969812073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG150.002218253470.024
AGTGGCC150.002218253470.063
AGCGAGT150.002218253470.070
GTTGATC200.006931415352.522
TCGTTAC200.006931415352.528
CTGTAGT200.006931415352.51
GATCGGG200.006931415352.525
TGGCGCT200.006931415352.513
TTCGTTA200.006931415352.527
CCAGCGA200.006931415352.568
GTGGCCA200.006931415352.564
TGCGCGG200.006931415352.55
GCCGTAT1550.049.6774254
CGTATCA1650.048.7878856
GTATCAT1600.048.12557
CATTAAA1650.046.66666861
GGAGCAA305.8505125E-446.6666641
ATCTCGG1600.045.937542
AGATCTC1600.045.937540
CGCCGTA1600.045.937553
Produced by FastQC (version 0.11.5)