FastQCFastQC Report
Wed 25 May 2016
SRR1780750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90301
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT1558317.256730268767786No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG22482.4894519440537755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15951.7663148802338844No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT12461.3798296807344326No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11951.323351900864885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC5540.613503726426064No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4920.544844464623869No Hit
CTTATACACATCTCCGCGCCCACGAGACTTTAATGCATCTCGTATGCCGT3650.40420371867421184No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT3480.38537779205102934Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC3360.37208890266995936No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3180.3521555685983544No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG2880.31893334514567945No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT2880.31893334514567945No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG2320.25691852803401954No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT2270.2513814907919071No Hit
CATAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT1670.18493704388655718No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT1660.18382963643813469No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGACGT1580.1749703768507547No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAATGCATCTCGTATGCCGT1470.16278889491810722No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC1370.15171482043388224No Hit
ATACCCATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT1200.13288889381069977Illumina PCR Primer Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTAAATGCATCTCGTATGCCGT1190.13178148636227727No Hit
TCAAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG1070.1184925969812073No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAAAGCATCTCGTATGCCGT1040.11517037463593979No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTACTGCATCTCGTATGCCGT1030.11406296718751731No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG970.10741852249698232No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC920.10188148525486981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATA150.002214840870.02714526
AATCTGC150.002214840870.02714551
TCCTGGT150.002219715269.9883744
GTGGGAT150.002219715269.9883714
GACAACG150.002219715269.9883718
GATCCCA252.8226186E-669.9883716
AGCATCT353.6670826E-969.9883734
ATCCCAC150.002219715269.9883717
TGGATGT150.002219715269.9883736
CCGAAAT150.002219715269.9883747
CTCCTGG150.002219715269.9883743
CACGTAA150.002219715269.9883739
GACGACA150.002219715269.9883746
TCCCACG207.895678E-569.9883718
ACGACAT150.002219715269.9883747
GGTAGTG150.002219715269.9883748
TGCCGTA150.002219715269.9883745
GACTTAA900.066.1367526
TATCGTA650.064.6046538
AGGGGGG21000.064.3226570
Produced by FastQC (version 0.11.5)