FastQCFastQC Report
Wed 25 May 2016
SRR1780749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34113
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2301567.46694808430803No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG3681.0787676252455076No Hit
TCTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGT520.152434555741213No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.14950312197695892No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460.13484595315568845No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.11432591680590977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCGC150.002206386870.012
CGAATCA200.006894617352.50000456
CCGAATC200.006894617352.50000455
CATTAAA604.7020876E-946.66666461
ATTAAAA604.7020876E-946.66666462
ATCATTA604.7020876E-946.66666459
TCATTAA604.7020876E-946.66666460
AGATCTC604.7020876E-946.66666440
TTAAAAA703.619789E-1045.063
TAGATCT551.2631426E-744.54545239
GTAGATC659.542418E-943.07692738
ATCTCGG659.542418E-943.07692742
GATCTCG659.542418E-943.07692741
GGTCGCC659.542418E-943.07692750
GTGGTCG602.5022018E-740.83333248
CTCGGTG701.8357241E-840.044
TGGTCGC556.5101704E-638.18181649
GTATCAT556.5101704E-638.18181657
CCGTATC556.5101704E-638.18181655
TCGCCGT556.5101704E-638.18181652