SRR1780749_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780749_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34113
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	12398	36.34391580922229	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	1659	4.863248614897547	No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	702	2.057866502506376	No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	700	2.0520036349778676	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	661	1.9376777181719578	No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT	512	1.5008940872980976	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	455	1.333802362735614	No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	269	0.788555682584352	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	238	0.697681235892475	No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT	234	0.6859555008354586	RNA PCR Primer, Index 34 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	162	0.47489226980916366	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT	139	0.4074692932313194	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT	132	0.38694925688154075	No Hit
CTAAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	120	0.3517720517104916	No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCG	101	0.2960748101896638	No Hit
CAAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	98	0.2872805088969015	No Hit
TCTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	97	0.2843490751326474	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT	86	0.2521033037258523	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT	85	0.24917186996159824	No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC	83	0.24330900243309003	No Hit
CATAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	83	0.24330900243309003	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT	77	0.22572039984756542	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC	69	0.20226892973353267	RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGA	68	0.19933749596927858	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT	67	0.1964060622050245	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	66	0.19347462844077037	No Hit
ATACCCATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT	60	0.1758860258552458	RNA PCR Primer, Index 34 (95% over 23bp)
CTCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	57	0.16709172456248353	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	56	0.1641602907982294	No Hit
CTAATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT	53	0.15536598950546712	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGGGATCTCGTATGCCGT	50	0.14657168821270483	No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	49	0.14364025444845074	No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	47	0.13777738691994254	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	45	0.13191451939143434	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT	42	0.12312021809867206	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACATGGGATCTCGTATGCCGT	42	0.12312021809867206	No Hit
CTTATAAACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	41	0.12018878433441796	No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTCATGGGATCTCGTATGCCGT	38	0.11139448304165567	No Hit
CTTAAACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT	38	0.11139448304165567	No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT	38	0.11139448304165567	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGCTCTCGTATGCCGT	37	0.1084630492774016	No Hit
TCAAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	36	0.10553161551314749	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCTT	20	7.818986E-5	70.00001	53
AACAAAT	20	7.818986E-5	70.00001	5
ACGTCAT	20	7.818986E-5	70.00001	47
CACGTAT	20	7.818986E-5	70.00001	39
TTCTGAA	20	7.818986E-5	70.00001	52
ACTCCGA	20	7.818986E-5	70.00001	11
TCTACTG	20	7.818986E-5	70.00001	50
AGTATGA	20	7.818986E-5	70.00001	41
TCAAATA	20	7.818986E-5	70.00001	1
AGAGCCC	20	7.818986E-5	70.00001	15
GACGTCA	20	7.818986E-5	70.00001	46
CCACATG	20	7.818986E-5	70.00001	28
GATCTAG	80	0.0	70.00001	36
TCTGAAT	20	7.818986E-5	70.00001	53
AAGAAAA	20	7.818986E-5	70.00001	58
CCTTATA	20	7.818986E-5	70.00001	1
GTCTACT	20	7.818986E-5	70.00001	49
TCGTATT	20	7.818986E-5	70.00001	40
TCCGTCT	20	7.818986E-5	70.00001	46
CAGCTAG	15	0.0022063868	70.0	54

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content