FastQCFastQC Report
Wed 25 May 2016
SRR1780748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4325.131860299358518No Hit
ATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTGGTC140.1663102874792112No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG130.15443098123069612No Hit
GCGTCAGATGTGTATAAGAGACAGGTATAAGAGACAGTGGGCTCGGAGAT130.15443098123069612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAA200.006715398352.512
Produced by FastQC (version 0.11.5)