FastQCFastQC Report
Wed 25 May 2016
SRR1780747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20478
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6553.1985545463424163No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG440.21486473288407068No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA150.002193701770.010
TCAGATG150.002193701770.04
CAGATGT150.002193701770.05
CGGTCAC150.002193701770.034
TGTATAA150.002193701770.011
CGTCAGA200.006855283452.4999962
ATAAGAG200.006855283452.49999614
GCAATAG200.006855283452.49999645
GTATAAG200.006855283452.49999612
AGATGTG200.006855283452.4999966
AGAGACA200.006855283452.49999617
GCGTCAG200.006855283452.4999961
Produced by FastQC (version 0.11.5)